A novel optimized silver nitrate staining method for visualizing the osteocyte lacuno-canalicular system

A 50% (w/v, equivalent to 2.943 mol/L) silver nitrate solution is commonly used to stain and characterize the osteocyte lacuno-canalicular system (LCS) in bone biology research. However, variability in reagent concentrations and types, along with inconsistent staining procedures, have limited the br...

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Main Authors: Jinlian Wu, Chunchun Xue, Qiang Li, Hongjin Wu, Jie Zhang, Chenglong Wang, Weiwei Dai, Libo Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Endocrinology
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Online Access:https://www.frontiersin.org/articles/10.3389/fendo.2025.1561576/full
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