Expression characteristics of CsESA1 in citrus and analysis of its interacting protein
The most damaging disease affecting citrus globally is Huanglongbing (HLB), primarily attributed to the infection by ‘Candidatus Liberibacter asiaticus’ (CaLas). Based on comparative transcriptome data, two cellulose synthase (CESA) genes responsive to CaLas infection induction were screened, and on...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Plant Signaling & Behavior |
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| Online Access: | http://dx.doi.org/10.1080/15592324.2024.2439249 |
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| _version_ | 1846095033004982272 |
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| author | Xiao He Huiying Wang Wei Wei Ziyue Han Jiaqi Zuo Qing He |
| author_facet | Xiao He Huiying Wang Wei Wei Ziyue Han Jiaqi Zuo Qing He |
| author_sort | Xiao He |
| collection | DOAJ |
| description | The most damaging disease affecting citrus globally is Huanglongbing (HLB), primarily attributed to the infection by ‘Candidatus Liberibacter asiaticus’ (CaLas). Based on comparative transcriptome data, two cellulose synthase (CESA) genes responsive to CaLas infection induction were screened, and one gene cloned with higher differential expression level was selected and named CsCESA1. we verified the interaction between CsCESA1 and citrus exopolysaccharide 2 (CsEPS2) proteins. Subcellular localization in tobacco indicated that both CsCESA1 and CsEPS2 proteins are primarily located in the nucleus and cytoplasm. RT-qPCR analysis indicated that the expression levels of CsCESA1 and CsEPS2 were associated with variety tolerance, tissue site, and symptom development. Furthermore, we generated CsCESA1 and CsEPS2 silencing plants and obtained CsCESA1 and CsEPS2 silencing and overexpressing hairy roots. The analysis of hormone content and gene expression also showed that CsCESA1 and CsEPS2 are involved in transcriptional regulation of genes involved in systemic acquired resistance (SAR) response. In conclusion, our results suggested that CsCESA1 and CsEPS2 could serve as potential resistance genes for HLB disease, offering insights into the plant’s defense mechanisms against HLB. |
| format | Article |
| id | doaj-art-8dc28e5314a0402d820cdf790b114842 |
| institution | Kabale University |
| issn | 1559-2324 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj-art-8dc28e5314a0402d820cdf790b1148422025-01-02T11:44:27ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23242025-12-0120110.1080/15592324.2024.24392492439249Expression characteristics of CsESA1 in citrus and analysis of its interacting proteinXiao He0Huiying Wang1Wei Wei2Ziyue Han3Jiaqi Zuo4Qing He5Chongqing Three Gorges Medical CollegeSouthwest UniversityChongqing Normal UniversityJinzhong UniversityInner Mongolia University for NationalitiesChongqing Three Gorges Medical CollegeThe most damaging disease affecting citrus globally is Huanglongbing (HLB), primarily attributed to the infection by ‘Candidatus Liberibacter asiaticus’ (CaLas). Based on comparative transcriptome data, two cellulose synthase (CESA) genes responsive to CaLas infection induction were screened, and one gene cloned with higher differential expression level was selected and named CsCESA1. we verified the interaction between CsCESA1 and citrus exopolysaccharide 2 (CsEPS2) proteins. Subcellular localization in tobacco indicated that both CsCESA1 and CsEPS2 proteins are primarily located in the nucleus and cytoplasm. RT-qPCR analysis indicated that the expression levels of CsCESA1 and CsEPS2 were associated with variety tolerance, tissue site, and symptom development. Furthermore, we generated CsCESA1 and CsEPS2 silencing plants and obtained CsCESA1 and CsEPS2 silencing and overexpressing hairy roots. The analysis of hormone content and gene expression also showed that CsCESA1 and CsEPS2 are involved in transcriptional regulation of genes involved in systemic acquired resistance (SAR) response. In conclusion, our results suggested that CsCESA1 and CsEPS2 could serve as potential resistance genes for HLB disease, offering insights into the plant’s defense mechanisms against HLB.http://dx.doi.org/10.1080/15592324.2024.2439249citruscscesa1cseps2huanglongbinghairy roots |
| spellingShingle | Xiao He Huiying Wang Wei Wei Ziyue Han Jiaqi Zuo Qing He Expression characteristics of CsESA1 in citrus and analysis of its interacting protein Plant Signaling & Behavior citrus cscesa1 cseps2 huanglongbing hairy roots |
| title | Expression characteristics of CsESA1 in citrus and analysis of its interacting protein |
| title_full | Expression characteristics of CsESA1 in citrus and analysis of its interacting protein |
| title_fullStr | Expression characteristics of CsESA1 in citrus and analysis of its interacting protein |
| title_full_unstemmed | Expression characteristics of CsESA1 in citrus and analysis of its interacting protein |
| title_short | Expression characteristics of CsESA1 in citrus and analysis of its interacting protein |
| title_sort | expression characteristics of csesa1 in citrus and analysis of its interacting protein |
| topic | citrus cscesa1 cseps2 huanglongbing hairy roots |
| url | http://dx.doi.org/10.1080/15592324.2024.2439249 |
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