TIRE-seq simplifies transcriptomics via integrated RNA capture and library preparation

TIRE-seq simplifies transcriptomics via integrated RNA capture and library preparation

Abstract RNA sequencing (RNA-seq) is widely used in biomedical research, advancing our understanding of gene expression across biological systems. Traditional methods require upstream RNA extraction from biological inputs, adding time and expense to workflows. We developed TIRE-seq (Turbocapture Int...

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Main Authors: Piper O’Keeffe, Yasmin Nouri, Hui Shi Saw, Zachery Moore, Tracey M. Baldwin, Sam W. Z. Olechnowicz, Jafar S. Jabbari, David McG Squire, Stephen Leslie, Changqing Wang, Yupei You, Matthew E. Ritchie, Ryan S. Cross, Misty R. Jenkins, Cindy Audiger, Shalin H. Naik, James R. Whittle, Saskia Freytag, Sarah A. Best, Peter F. Hickey, Daniela Amann-Zalcenstein, Rory Bowden, Daniel V. Brown
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Subjects:
Transcriptomics
RNA
Extraction
High throughput
Online Access:https://doi.org/10.1038/s41598-025-98282-8
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https://doi.org/10.1038/s41598-025-98282-8

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