Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis
Objective To investigate the effect of diammonium glycyrrhizinate (DG) on pulmonary injury of rats with pulmonary tuberculosis. Methods The rat models of pulmonary tuberculosis were constructed and then the animals were randomly divided into model group, diammonium glycyrrhizinate treatment groups (...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2024-11-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-11-1544.pdf |
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| author | CAO Peiqian, WANG Zhigang, MIAO Xuehong |
| author_facet | CAO Peiqian, WANG Zhigang, MIAO Xuehong |
| author_sort | CAO Peiqian, WANG Zhigang, MIAO Xuehong |
| collection | DOAJ |
| description | Objective To investigate the effect of diammonium glycyrrhizinate (DG) on pulmonary injury of rats with pulmonary tuberculosis. Methods The rat models of pulmonary tuberculosis were constructed and then the animals were randomly divided into model group, diammonium glycyrrhizinate treatment groups (low-dose, medium-dose and high-dose) groups, high dose of diammonium glycyrrhizinate plus peroxisome proliferator-activated receptor γ(PPARγ) inhibitor group (H-DG+GW9662 group), and another 18 rats were selected as control group. The colony count of Mycobacterium tuberculosis(Mtb) in lung tissue was detected. HE staining microscopy was applied to detect lung histopathology. TUNEL was applied to detect apoptosis of lung tissue cells. ELISA was applied to detect serum level of inflammatory factors. Western blot was applied to measure PPARγ, phosphorylated p38 mitogen-activated protein kinase(p-p38MAPK) and p38 mitogen-activated protein kinase (p38MAPK) in lung tissue. Results Compared with the control group, the lung tissue structure in model group was severely damaged with a large number of proliferative tuberculosis nodules, changes of alveolar morphology, inflammatory cell infiltration and even caseous necrosis were found, and the number of tuberculosis colonies, apoptosis rate, TNF-α, IL-6, IFN-γ, COX-2 levels, and p-p38MAPK/p38MAPK expression were all increased, while PPARγ expression was decreased(P＜0.05). In L-DG, M-DG, H-DG groups improvement of lung tissue structure, alveolar morphology, inflammatory cell infiltration, and caseous necrosis were found as compared to the model group, while the counting number of tuberculosis colonies decreased and rate of cell apoptosis decreased. The level of TNF-α, IL-6, IFN-γ, COX-2 and expression of p-p38MAPK/p38MAPK reduced, the expression of PPARγ all increased. The H-DG group showed the most significant changes(P＜0.05). GW9662 treatment significantly reversed the improvement of DG on pulmonary injury in rats with pulmonary tuberculosis. Conclusions DG improves lung injury in rats with pulmonary tuberculosis and its mechanism is potentially related to the activation of PPARγ pathway and inhibition of p38MAPK pathway. |
| format | Article |
| id | doaj-art-87ebe69346a444249eb4d91c60360105 |
| institution | Kabale University |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2024-11-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-87ebe69346a444249eb4d91c603601052024-11-12T06:26:23ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252024-11-0144111544155010.16352/j.issn.1001-6325.2024.11.1544Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosisCAO Peiqian, WANG Zhigang, MIAO Xuehong0Department of Juvenile Tuberculosis, the Sixth People's Hospital of Zhengzhou, Zhengzhou 450000, ChinaObjective To investigate the effect of diammonium glycyrrhizinate (DG) on pulmonary injury of rats with pulmonary tuberculosis. Methods The rat models of pulmonary tuberculosis were constructed and then the animals were randomly divided into model group, diammonium glycyrrhizinate treatment groups (low-dose, medium-dose and high-dose) groups, high dose of diammonium glycyrrhizinate plus peroxisome proliferator-activated receptor γ(PPARγ) inhibitor group (H-DG+GW9662 group), and another 18 rats were selected as control group. The colony count of Mycobacterium tuberculosis(Mtb) in lung tissue was detected. HE staining microscopy was applied to detect lung histopathology. TUNEL was applied to detect apoptosis of lung tissue cells. ELISA was applied to detect serum level of inflammatory factors. Western blot was applied to measure PPARγ, phosphorylated p38 mitogen-activated protein kinase(p-p38MAPK) and p38 mitogen-activated protein kinase (p38MAPK) in lung tissue. Results Compared with the control group, the lung tissue structure in model group was severely damaged with a large number of proliferative tuberculosis nodules, changes of alveolar morphology, inflammatory cell infiltration and even caseous necrosis were found, and the number of tuberculosis colonies, apoptosis rate, TNF-α, IL-6, IFN-γ, COX-2 levels, and p-p38MAPK/p38MAPK expression were all increased, while PPARγ expression was decreased(P＜0.05). In L-DG, M-DG, H-DG groups improvement of lung tissue structure, alveolar morphology, inflammatory cell infiltration, and caseous necrosis were found as compared to the model group, while the counting number of tuberculosis colonies decreased and rate of cell apoptosis decreased. The level of TNF-α, IL-6, IFN-γ, COX-2 and expression of p-p38MAPK/p38MAPK reduced, the expression of PPARγ all increased. The H-DG group showed the most significant changes(P＜0.05). GW9662 treatment significantly reversed the improvement of DG on pulmonary injury in rats with pulmonary tuberculosis. Conclusions DG improves lung injury in rats with pulmonary tuberculosis and its mechanism is potentially related to the activation of PPARγ pathway and inhibition of p38MAPK pathway.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-11-1544.pdfdiammonium glycyrrhizinate|peroxisome proliferators-activated receptor γ/p38 mitogen-activated protein kinase pathway|pulmonary tuberculosis|lung injury |
| spellingShingle | CAO Peiqian, WANG Zhigang, MIAO Xuehong Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis Jichu yixue yu linchuang diammonium glycyrrhizinate|peroxisome proliferators-activated receptor γ/p38 mitogen-activated protein kinase pathway|pulmonary tuberculosis|lung injury |
| title | Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis |
| title_full | Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis |
| title_fullStr | Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis |
| title_full_unstemmed | Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis |
| title_short | Diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis |
| title_sort | diammonium glycyrrhizinate alleviates lung injury in rat models with lung tuberculosis |
| topic | diammonium glycyrrhizinate|peroxisome proliferators-activated receptor γ/p38 mitogen-activated protein kinase pathway|pulmonary tuberculosis|lung injury |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-11-1544.pdf |
| work_keys_str_mv | AT caopeiqianwangzhigangmiaoxuehong diammoniumglycyrrhizinatealleviateslunginjuryinratmodelswithlungtuberculosis |