Postbiotic Potential of Lactic Acid Bacteria Strains in Functional Minimally Processed Oranges
The global market for fresh, ready-to-eat products has grown rapidly, leading to some microbiological safety concerns. Lactic acid bacteria (LAB), along with their metabolites, represent a green alternative to chemical preservatives. For this purpose, the cell-free supernatant of LAB strains previou...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/15/12/6736 |
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| Summary: | The global market for fresh, ready-to-eat products has grown rapidly, leading to some microbiological safety concerns. Lactic acid bacteria (LAB), along with their metabolites, represent a green alternative to chemical preservatives. For this purpose, the cell-free supernatant of LAB strains previously isolated from fruits and their potential antibacterial effects against pathogens commonly found in minimally processed fruit were evaluated. Based on the preliminary results, a mix of cell-free supernatants (CFSs) was applied as a postbiotic solution in minimally processed orange slices packed in a passive atmosphere. Different pathogenic strains were intentionally inoculated to evaluate their antimicrobial effect, and their trend was monitored. Microbiological and physico-chemical analyses were carried out at different times (3, 8, and 10 days) during cold storage. The CFSs obtained from <i>Lactiplantibacillus plantarum</i> and <i>Leuconostoc mesenteroides</i> strains highlighted an antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> in both in vitro and in vivo tests, showing a reduction of 1 Log CFU/mL for <i>E. coli</i> and the disappearance of vital <i>S. aureus</i>. In conclusion, the CFSs can be proposed as an eco-friendly biopreservative for orange slices with a clean label, although their stability needs to be evaluated and their limits of application need to be ruled by specific legislation. |
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| ISSN: | 2076-3417 |