Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric Ulcers
Objective: Gastric ulcer is frequently observed among the gastrointestinal diseases and is induced by various factors. Alpha-mangostin (α-MG) has antioxidant and anti-inflammatory properties and may prevent gastric ulcers. This study was conducted to evaluate the healing effect of α-MG against gastr...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2025-04-01
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| Series: | Gazi Medical Journal |
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| Online Access: | https://gazimedj.com/articles/alpha-mangostin-provides-protection-from-mucosal-damage-via-prostaglandin-e2-in-indomethacin-and-ethanol-induced-gastric-ulcers/doi/gmj.2025.3895 |
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| author | Ersen Eraslan Burak Bircan Ayhan Tanyeli Mustafa Can Güler Yasin Bayır Selim Çomaklı |
| author_facet | Ersen Eraslan Burak Bircan Ayhan Tanyeli Mustafa Can Güler Yasin Bayır Selim Çomaklı |
| author_sort | Ersen Eraslan |
| collection | DOAJ |
| description | Objective: Gastric ulcer is frequently observed among the gastrointestinal diseases and is induced by various factors. Alpha-mangostin (α-MG) has antioxidant and anti-inflammatory properties and may prevent gastric ulcers. This study was conducted to evaluate the healing effect of α-MG against gastric ulcer caused by indomethacin (Ind) and ethanol (Eth) in rats.
Methods: Wistar albino male rats were used to establish the experimental model. Seven groups were formed, as group I sham, group II (5 mL/kg Eth), group III (100 mg/kg Ind), group IV (Eth + Lansoprazole (Lans) 30 mg/kg), group V (Ind + Lans 30 mg/kg), group VI (Eth + α-MG 10 mg/kg), and group VII (Ind + α-MG 10 mg/kg) (n=10). Cytokines; VEGF-A; NOS2/iNOS; PGE2 levels were analyzed by the ELISA method. Besides, the general appearance of the gastric tissues was evaluated by hematoxylin-eosin staining, COX-1, COX-2, NF-κB, and caspase-3 levels were measured immunohistochemical (IHC).
Results: Cytokine levels decreased in the treatment groups compared to the ulcer groups. There was a decrease in VEGF-A and NOS2/iNOS levels in the α-MG administered groups. The reduction in PGE2 levels in the gastric ulcer groups was counteracted by an increase in both the Lans and α-MG administered groups. In the IHC results, while COX-1, COX-2, NF-κB, and caspase-3 levels were increased in gastric ulcer groups, significant decreases were observed in Lans and α-MG groups.
Conclusion: As a result, α-MG eased inflammation and increased PGE2 levels. It reduced the levels of COX-1, COX-2, NF-κB, and caspase-3. As a result of these data, α-MG may be a potential therapeutic agent against gastric ulcer. |
| format | Article |
| id | doaj-art-84dc2a4e96b5452a9dff91e535e29ed9 |
| institution | OA Journals |
| issn | 2147-2092 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Gazi Medical Journal |
| spelling | doaj-art-84dc2a4e96b5452a9dff91e535e29ed92025-08-20T02:27:32ZengGalenos Publishing HouseGazi Medical Journal2147-20922025-04-0136213614310.12996/gmj.2025.3895Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric UlcersErsen Eraslan0https://orcid.org/0000-0003-2424-2269Burak Bircan1https://orcid.org/0000-0003-1141-069XAyhan Tanyeli2https://orcid.org/0000-0002-0095-0917Mustafa Can Güler3https://orcid.org/0000-0001-8588-1035Yasin Bayır4https://orcid.org/0000-0003-3562-6727Selim Çomaklı5https://orcid.org/0000-0002-8744-7686Department of Physiology, Yozgat Bozok University Faculty of Medicine, Yozgat, TürkiyeDepartment of Anesthesia, Osmaniye Korkut Ata University Healthcare Vocational School, Osmaniye, TürkiyeDepartment of Physiology, Atatürk University Faculty of Medicine, Erzurum, TürkiyeDepartment of Physiology, Atatürk University Faculty of Medicine, Erzurum, TürkiyeDepartment of Biochemistry, Atatürk University Faculty of Medicine, Erzurum, TürkiyeDepartment of Pathology, Atatürk University Faculty of Medicine, Erzurum, TürkiyeObjective: Gastric ulcer is frequently observed among the gastrointestinal diseases and is induced by various factors. Alpha-mangostin (α-MG) has antioxidant and anti-inflammatory properties and may prevent gastric ulcers. This study was conducted to evaluate the healing effect of α-MG against gastric ulcer caused by indomethacin (Ind) and ethanol (Eth) in rats. Methods: Wistar albino male rats were used to establish the experimental model. Seven groups were formed, as group I sham, group II (5 mL/kg Eth), group III (100 mg/kg Ind), group IV (Eth + Lansoprazole (Lans) 30 mg/kg), group V (Ind + Lans 30 mg/kg), group VI (Eth + α-MG 10 mg/kg), and group VII (Ind + α-MG 10 mg/kg) (n=10). Cytokines; VEGF-A; NOS2/iNOS; PGE2 levels were analyzed by the ELISA method. Besides, the general appearance of the gastric tissues was evaluated by hematoxylin-eosin staining, COX-1, COX-2, NF-κB, and caspase-3 levels were measured immunohistochemical (IHC). Results: Cytokine levels decreased in the treatment groups compared to the ulcer groups. There was a decrease in VEGF-A and NOS2/iNOS levels in the α-MG administered groups. The reduction in PGE2 levels in the gastric ulcer groups was counteracted by an increase in both the Lans and α-MG administered groups. In the IHC results, while COX-1, COX-2, NF-κB, and caspase-3 levels were increased in gastric ulcer groups, significant decreases were observed in Lans and α-MG groups. Conclusion: As a result, α-MG eased inflammation and increased PGE2 levels. It reduced the levels of COX-1, COX-2, NF-κB, and caspase-3. As a result of these data, α-MG may be a potential therapeutic agent against gastric ulcer.https://gazimedj.com/articles/alpha-mangostin-provides-protection-from-mucosal-damage-via-prostaglandin-e2-in-indomethacin-and-ethanol-induced-gastric-ulcers/doi/gmj.2025.3895gastric ulcerindomethacinethanolalpha-mangostinrat |
| spellingShingle | Ersen Eraslan Burak Bircan Ayhan Tanyeli Mustafa Can Güler Yasin Bayır Selim Çomaklı Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric Ulcers Gazi Medical Journal gastric ulcer indomethacin ethanol alpha-mangostin rat |
| title | Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric Ulcers |
| title_full | Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric Ulcers |
| title_fullStr | Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric Ulcers |
| title_full_unstemmed | Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric Ulcers |
| title_short | Alpha-Mangostin Provides Protection from Mucosal Damage via Prostaglandin E2 in Indomethacin and Ethanol-Induced Gastric Ulcers |
| title_sort | alpha mangostin provides protection from mucosal damage via prostaglandin e2 in indomethacin and ethanol induced gastric ulcers |
| topic | gastric ulcer indomethacin ethanol alpha-mangostin rat |
| url | https://gazimedj.com/articles/alpha-mangostin-provides-protection-from-mucosal-damage-via-prostaglandin-e2-in-indomethacin-and-ethanol-induced-gastric-ulcers/doi/gmj.2025.3895 |
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