Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship study

In this study, the researchers produced a pear paste using typical Dangshan pear fruits. The mechanism that enables the paste to treat cough and related inflammatory responses was systematically investigated using an ammonia-induced mice cough model. The pear paste and its polar extracts relieved co...

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Main Authors: Min Xu, Yuhui Yu, Huixin Xu, Meiqing Li
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Journal of Functional Foods
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Online Access:http://www.sciencedirect.com/science/article/pii/S1756464624004936
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author Min Xu
Yuhui Yu
Huixin Xu
Meiqing Li
author_facet Min Xu
Yuhui Yu
Huixin Xu
Meiqing Li
author_sort Min Xu
collection DOAJ
description In this study, the researchers produced a pear paste using typical Dangshan pear fruits. The mechanism that enables the paste to treat cough and related inflammatory responses was systematically investigated using an ammonia-induced mice cough model. The pear paste and its polar extracts relieved cough symptoms, as evidenced by 44.0%, 53.4%, and 47.2% decreases in cough frequency and 21.0%, 43.3%, and 53.4% increases in latent period for pear paste, ethyl acetate extract (EAE) and n-butanol extract (WBE), respectively. In addition, the paste and its polar extracts reduced lung injuries, including alveolar dilatation, alveolar wall damage, and airway thickening. The two polar extracts exhibited anti-inflammatory effects in the airway through the suppression of a Th1 cytokine (IFN-γ, significantly suppressed by 15.49% and 16.33% for EAE and WBE, separately). A spectrum-effect relationship analysis was conducted to identify arbutin, protocatechuic acid, chlorogenic acid and naringin as the primary bioactive substances contributing to the antitussive and anti-inflammatory activities of pear paste.
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institution Kabale University
issn 1756-4646
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publisher Elsevier
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series Journal of Functional Foods
spelling doaj-art-8173190983d74554ae19ff78d190a95d2024-11-16T05:10:38ZengElsevierJournal of Functional Foods1756-46462024-11-01122106491Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship studyMin Xu0Yuhui Yu1Huixin Xu2Meiqing Li3Anhui Vocational College of Grain Engineering, Hefei 230036, PR ChinaState Key Laboratory of Tea Biology and Utilization, Anhui Agriculture University, Hefei 230036, PR ChinaState Key Laboratory of Tea Biology and Utilization, Anhui Agriculture University, Hefei 230036, PR ChinaState Key Laboratory of Tea Biology and Utilization, Anhui Agriculture University, Hefei 230036, PR China; Corresponding author.In this study, the researchers produced a pear paste using typical Dangshan pear fruits. The mechanism that enables the paste to treat cough and related inflammatory responses was systematically investigated using an ammonia-induced mice cough model. The pear paste and its polar extracts relieved cough symptoms, as evidenced by 44.0%, 53.4%, and 47.2% decreases in cough frequency and 21.0%, 43.3%, and 53.4% increases in latent period for pear paste, ethyl acetate extract (EAE) and n-butanol extract (WBE), respectively. In addition, the paste and its polar extracts reduced lung injuries, including alveolar dilatation, alveolar wall damage, and airway thickening. The two polar extracts exhibited anti-inflammatory effects in the airway through the suppression of a Th1 cytokine (IFN-γ, significantly suppressed by 15.49% and 16.33% for EAE and WBE, separately). A spectrum-effect relationship analysis was conducted to identify arbutin, protocatechuic acid, chlorogenic acid and naringin as the primary bioactive substances contributing to the antitussive and anti-inflammatory activities of pear paste.http://www.sciencedirect.com/science/article/pii/S1756464624004936Pear pasteAntitussive effectInflammation responseSpectrum-effect relationship
spellingShingle Min Xu
Yuhui Yu
Huixin Xu
Meiqing Li
Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship study
Journal of Functional Foods
Pear paste
Antitussive effect
Inflammation response
Spectrum-effect relationship
title Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship study
title_full Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship study
title_fullStr Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship study
title_full_unstemmed Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship study
title_short Identification of active substances in pear paste for the treatment of cough in mice using spectrum-effect relationship study
title_sort identification of active substances in pear paste for the treatment of cough in mice using spectrum effect relationship study
topic Pear paste
Antitussive effect
Inflammation response
Spectrum-effect relationship
url http://www.sciencedirect.com/science/article/pii/S1756464624004936
work_keys_str_mv AT minxu identificationofactivesubstancesinpearpasteforthetreatmentofcoughinmiceusingspectrumeffectrelationshipstudy
AT yuhuiyu identificationofactivesubstancesinpearpasteforthetreatmentofcoughinmiceusingspectrumeffectrelationshipstudy
AT huixinxu identificationofactivesubstancesinpearpasteforthetreatmentofcoughinmiceusingspectrumeffectrelationshipstudy
AT meiqingli identificationofactivesubstancesinpearpasteforthetreatmentofcoughinmiceusingspectrumeffectrelationshipstudy