Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA Pathway
Perineural invasion (PNI) is an important factor leading to the recurrence of pancreatic cancer (PanCa). The NGF–TrkA pathway is related to PNI progression. Ganoderma spore lipid (GSL) is a drug with anti-cancer properties. In this study, we find out whether GSL can prevent PNI of PanCa by inhibitin...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2025-07-01
|
| Series: | Journal of Evidence-Based Integrative Medicine |
| Online Access: | https://doi.org/10.1177/2515690X251356473 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849416000378765312 |
|---|---|
| author | Haohui Lin MS Manhon Chung MD Yi Yang MS Li Zhang MS Xiaohua Pan MD Sa Cai MD, PhD Yu Pan PhD |
| author_facet | Haohui Lin MS Manhon Chung MD Yi Yang MS Li Zhang MS Xiaohua Pan MD Sa Cai MD, PhD Yu Pan PhD |
| author_sort | Haohui Lin MS |
| collection | DOAJ |
| description | Perineural invasion (PNI) is an important factor leading to the recurrence of pancreatic cancer (PanCa). The NGF–TrkA pathway is related to PNI progression. Ganoderma spore lipid (GSL) is a drug with anti-cancer properties. In this study, we find out whether GSL can prevent PNI of PanCa by inhibiting NGF–TrkA pathway. In vitro , wound healing assays, transwell-based assays and three-dimensional tumor-nerve cell co-culture system showed that GSL significantly inhibited the migration and invasion capacity of PanCa cells. Inhibiting the NGF–TrkA pathway is considered an effective approach for treating PanCa. We showed that GSL effectively inhibited NGF-TrkA pathway via immunofluorescence assays and western blotting analysis. The supplement of recombinant NGF reversed the GSL inhibitory effect on the migration and invasion of PANC-1. In vivo , a sciatic nerve invasion animal model was constructed using BALB/c mice. GSL significantly suppressed tumor growth and suppressed the expression of TrkA, NGF, and vimentin and upregulated the level of the epithelial marker E-Cadherin. Moreover, GSL reduced the expression of S100 and PGP9.5. These findings suggested that GSL effectively inhibited the PNI of PanCa cells by downregulating the NGF–TrkA pathway, which may provide a new adjuvant for PanCa treatment. |
| format | Article |
| id | doaj-art-7f89107c4c5a47bdbfdcf908dece60fb |
| institution | Kabale University |
| issn | 2515-690X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Evidence-Based Integrative Medicine |
| spelling | doaj-art-7f89107c4c5a47bdbfdcf908dece60fb2025-08-20T03:33:18ZengSAGE PublishingJournal of Evidence-Based Integrative Medicine2515-690X2025-07-013010.1177/2515690X251356473Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA PathwayHaohui Lin MS0Manhon Chung MD1Yi Yang MS2Li Zhang MS3Xiaohua Pan MD4Sa Cai MD, PhD5Yu Pan PhD6 Laboratory of Regenerative Medicine, The 2nd Affiliated Hospital of Shenzhen University, Medical School, , Shenzhen, China Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, , Shanghai, China Laboratory of Regenerative Medicine, The 2nd Affiliated Hospital of Shenzhen University, Medical School, , Shenzhen, China Laboratory of Regenerative Medicine, The 2nd Affiliated Hospital of Shenzhen University, Medical School, , Shenzhen, China Laboratory of Regenerative Medicine, The 2nd Affiliated Hospital of Shenzhen University, Medical School, , Shenzhen, China Laboratory of Regenerative Medicine, The 2nd Affiliated Hospital of Shenzhen University, Medical School, , Shenzhen, China Laboratory of Regenerative Medicine, The 2nd Affiliated Hospital of Shenzhen University, Medical School, , Shenzhen, ChinaPerineural invasion (PNI) is an important factor leading to the recurrence of pancreatic cancer (PanCa). The NGF–TrkA pathway is related to PNI progression. Ganoderma spore lipid (GSL) is a drug with anti-cancer properties. In this study, we find out whether GSL can prevent PNI of PanCa by inhibiting NGF–TrkA pathway. In vitro , wound healing assays, transwell-based assays and three-dimensional tumor-nerve cell co-culture system showed that GSL significantly inhibited the migration and invasion capacity of PanCa cells. Inhibiting the NGF–TrkA pathway is considered an effective approach for treating PanCa. We showed that GSL effectively inhibited NGF-TrkA pathway via immunofluorescence assays and western blotting analysis. The supplement of recombinant NGF reversed the GSL inhibitory effect on the migration and invasion of PANC-1. In vivo , a sciatic nerve invasion animal model was constructed using BALB/c mice. GSL significantly suppressed tumor growth and suppressed the expression of TrkA, NGF, and vimentin and upregulated the level of the epithelial marker E-Cadherin. Moreover, GSL reduced the expression of S100 and PGP9.5. These findings suggested that GSL effectively inhibited the PNI of PanCa cells by downregulating the NGF–TrkA pathway, which may provide a new adjuvant for PanCa treatment.https://doi.org/10.1177/2515690X251356473 |
| spellingShingle | Haohui Lin MS Manhon Chung MD Yi Yang MS Li Zhang MS Xiaohua Pan MD Sa Cai MD, PhD Yu Pan PhD Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA Pathway Journal of Evidence-Based Integrative Medicine |
| title | Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA Pathway |
| title_full | Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA Pathway |
| title_fullStr | Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA Pathway |
| title_full_unstemmed | Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA Pathway |
| title_short | Ganoderma Spore Lipids Inhibit Perineural Invasion in Pancreatic Cancer by Downregulating NGF-TrkA Pathway |
| title_sort | ganoderma spore lipids inhibit perineural invasion in pancreatic cancer by downregulating ngf trka pathway |
| url | https://doi.org/10.1177/2515690X251356473 |
| work_keys_str_mv | AT haohuilinms ganodermasporelipidsinhibitperineuralinvasioninpancreaticcancerbydownregulatingngftrkapathway AT manhonchungmd ganodermasporelipidsinhibitperineuralinvasioninpancreaticcancerbydownregulatingngftrkapathway AT yiyangms ganodermasporelipidsinhibitperineuralinvasioninpancreaticcancerbydownregulatingngftrkapathway AT lizhangms ganodermasporelipidsinhibitperineuralinvasioninpancreaticcancerbydownregulatingngftrkapathway AT xiaohuapanmd ganodermasporelipidsinhibitperineuralinvasioninpancreaticcancerbydownregulatingngftrkapathway AT sacaimdphd ganodermasporelipidsinhibitperineuralinvasioninpancreaticcancerbydownregulatingngftrkapathway AT yupanphd ganodermasporelipidsinhibitperineuralinvasioninpancreaticcancerbydownregulatingngftrkapathway |