Identification of Kunitz inhibitor from Cassia obtusifolia L. and its inhibitory effect against Pieris rapae proteases

A trypsin inhibitor from Cassia obtusifolia seeds, a well known Chinese herb, was isolated to apparent homogeneity by a combination of distilled water extraction, ammonium sulfate precipitation, Sepharose 4B-trypsin affinity and Sephadex G-75 chromatography. SDS-PAGE and MALDI-TOF analyses show that...

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Bibliographic Details
Main Authors: LIAO Hai, REN Wei, ZHAO Xiao-jun, DU Lin-fang
Format: Article
Language:English
Published: Zhejiang University Press 2008-01-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.005
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