Effect of a garlic drench on Galectin gene expression in ovine whole blood

Garlic, known for its immune-modulating and antibiotic properties, contains lectins that possess antimicrobial and immunomodulatory effects. Galectins (Gals), which bind β-galactosides, play a role in modulating immunity and pathological processes. It is hypothesized that garlic’s lectin components...

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Main Authors: SreeNavya Inupala, Md. Rasel Uzzaman, Priyanka Pande, Sowmya Jagana, Mulumebet Worku
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Animal Biotechnology
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/10495398.2024.2344208
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author SreeNavya Inupala
Md. Rasel Uzzaman
Priyanka Pande
Sowmya Jagana
Mulumebet Worku
author_facet SreeNavya Inupala
Md. Rasel Uzzaman
Priyanka Pande
Sowmya Jagana
Mulumebet Worku
author_sort SreeNavya Inupala
collection DOAJ
description Garlic, known for its immune-modulating and antibiotic properties, contains lectins that possess antimicrobial and immunomodulatory effects. Galectins (Gals), which bind β-galactosides, play a role in modulating immunity and pathological processes. It is hypothesized that garlic’s lectin components interfere with animal lectins. St. Croix sheep, known for their resistance to parasites and adaptability, are influenced by dietary supplements for innate immunity. This study evaluated the impact of garlic drench on Galectin gene expression in St. Croix sheep. Adult non-lactating ewes received either garlic juice concentrate or sterile distilled water for four weeks. Blood samples were collected, and plasma and whole blood cells were separated. Galectin secretion was assessed using a Sheep-specific ELISA, while Galectin gene transcription was analyzed through real-time PCR. Garlic administration upregulated LGALS-3 gene expression and significantly increased total plasma protein concentration. Garlic supplementation also affected Galectin secretion, with Gal-1, Gal-3, and Gal-9 showing differential effects.
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institution Kabale University
issn 1049-5398
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language English
publishDate 2024-12-01
publisher Taylor & Francis Group
record_format Article
series Animal Biotechnology
spelling doaj-art-74a684a5586342a699c331c9b747dd952024-12-31T18:28:12ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2344208Effect of a garlic drench on Galectin gene expression in ovine whole bloodSreeNavya Inupala0Md. Rasel Uzzaman1Priyanka Pande2Sowmya Jagana3Mulumebet Worku4Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USAGarlic, known for its immune-modulating and antibiotic properties, contains lectins that possess antimicrobial and immunomodulatory effects. Galectins (Gals), which bind β-galactosides, play a role in modulating immunity and pathological processes. It is hypothesized that garlic’s lectin components interfere with animal lectins. St. Croix sheep, known for their resistance to parasites and adaptability, are influenced by dietary supplements for innate immunity. This study evaluated the impact of garlic drench on Galectin gene expression in St. Croix sheep. Adult non-lactating ewes received either garlic juice concentrate or sterile distilled water for four weeks. Blood samples were collected, and plasma and whole blood cells were separated. Galectin secretion was assessed using a Sheep-specific ELISA, while Galectin gene transcription was analyzed through real-time PCR. Garlic administration upregulated LGALS-3 gene expression and significantly increased total plasma protein concentration. Garlic supplementation also affected Galectin secretion, with Gal-1, Gal-3, and Gal-9 showing differential effects.https://www.tandfonline.com/doi/10.1080/10495398.2024.2344208GarlicGalectinssheep
spellingShingle SreeNavya Inupala
Md. Rasel Uzzaman
Priyanka Pande
Sowmya Jagana
Mulumebet Worku
Effect of a garlic drench on Galectin gene expression in ovine whole blood
Animal Biotechnology
Garlic
Galectins
sheep
title Effect of a garlic drench on Galectin gene expression in ovine whole blood
title_full Effect of a garlic drench on Galectin gene expression in ovine whole blood
title_fullStr Effect of a garlic drench on Galectin gene expression in ovine whole blood
title_full_unstemmed Effect of a garlic drench on Galectin gene expression in ovine whole blood
title_short Effect of a garlic drench on Galectin gene expression in ovine whole blood
title_sort effect of a garlic drench on galectin gene expression in ovine whole blood
topic Garlic
Galectins
sheep
url https://www.tandfonline.com/doi/10.1080/10495398.2024.2344208
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AT mdraseluzzaman effectofagarlicdrenchongalectingeneexpressioninovinewholeblood
AT priyankapande effectofagarlicdrenchongalectingeneexpressioninovinewholeblood
AT sowmyajagana effectofagarlicdrenchongalectingeneexpressioninovinewholeblood
AT mulumebetworku effectofagarlicdrenchongalectingeneexpressioninovinewholeblood