AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIA
Background: The protective effect of α-thalassemia, a common hematological disorder in Southeast Asia, against Plasmodium falciparum malaria has been well established. However, there are much less well understood the effect of α-thalassemia against P.vivax. Here, we aimed to investigate the proporti...
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PAGEPress Publications
2018-02-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
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| Online Access: | https://www.mjhid.org/index.php/mjhid/article/view/3083 |
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| author | Suparak Para Puncharee Mungkalasut Makamas Chanda Issarang Nuchprayoon Srivicha Krundsood Chalisa Louicharoen Cheepsunthorn |
| author_facet | Suparak Para Puncharee Mungkalasut Makamas Chanda Issarang Nuchprayoon Srivicha Krundsood Chalisa Louicharoen Cheepsunthorn |
| author_sort | Suparak Para |
| collection | DOAJ |
| description | Background: The protective effect of α-thalassemia, a common hematological disorder in Southeast Asia, against Plasmodium falciparum malaria has been well established. However, there are much less well understood the effect of α-thalassemia against P.vivax. Here, we aimed to investigate the proportion of α-thalassemia including the effect of α-thalassemia and HbE on the parasitemia of P.vivax in Southeast Asian malaria patients in Thailand.
Methods: A total of 210 malaria patients, admitted to the Hospital for Tropical Diseases, Thailand during 2011-2012, consist of 159 Myanmeses, 13 Karens, 26 Thais, 3 Mons, 3 Laotians, and 6 Cambodians were recruited. Plasmodium spp. and parasite densities were determined. Group of deletion mutation (--SEA, -α3.7, -α4.2deletion) and substitution mutation (HbCS and HbE) were genotyped using multiplex gap-PCR and PCR-RFLP, respectively.
Results: In our malaria patients, 17/210 homozygous and 74/210 heterozygous -α3.7 deletion were found. Only 3/210 heterozygous -α4.2 deletion and 2/210 heterozygous--SEA deletion were detected. HbE is frequently found with 6/210 homozygote and 35/210 heterozygote. The most common thalassemia allele frequencies in Myanmar population were -α3.7 deletion (0.282), followed by HbE (0.101), HbCS (0.016), -α4.2 deletion (0.009), and --SEA deletion (0.003). Only density of P.vivax in α-thalassemia trait patients (-α3.7/-α3.7, --SEA/αα, -α3.7/-α4.2) but not in silent α-thalassemia (-α3.7/αα, -α4.2/αα, ααCS/αα) were significantly higher compared with non α-thalassemia patients (p=0.027). Density of P.falciparum significantly increased in heterozygous HbE patients (p=0.046).
Conclusions: Alpha-thalassemia trait is associated with high level of P.vivax parasitemia in malaria patients in Southeast Asia. |
| format | Article |
| id | doaj-art-72cf2d8846634c9b804d14568702bd5d |
| institution | Kabale University |
| issn | 2035-3006 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj-art-72cf2d8846634c9b804d14568702bd5d2025-01-02T08:53:25ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062018-02-01101e2018015e201801510.4084/mjhid.2018.0151739AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIASuparak Para0Puncharee Mungkalasut1Makamas Chanda2Issarang Nuchprayoon3Srivicha Krundsood4Chalisa Louicharoen Cheepsunthorn5Medical Biochemistry Program, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University,Medical Biochemistry Program, Department of Biochemistry, Faculty of Medicine, Chulalongkorn UniversityBiomedical Science Program, Graduate School, Chulalongkorn UniversityDepartment of Pediatrics, Faculty of Medicine, Chulalongkorn UniversityDepartment of Tropical Hygiene and Clinical Malaria Research Unit, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Biochemistry, Faculty of Medicine, Chulalongkorn UniversityBackground: The protective effect of α-thalassemia, a common hematological disorder in Southeast Asia, against Plasmodium falciparum malaria has been well established. However, there are much less well understood the effect of α-thalassemia against P.vivax. Here, we aimed to investigate the proportion of α-thalassemia including the effect of α-thalassemia and HbE on the parasitemia of P.vivax in Southeast Asian malaria patients in Thailand. Methods: A total of 210 malaria patients, admitted to the Hospital for Tropical Diseases, Thailand during 2011-2012, consist of 159 Myanmeses, 13 Karens, 26 Thais, 3 Mons, 3 Laotians, and 6 Cambodians were recruited. Plasmodium spp. and parasite densities were determined. Group of deletion mutation (--SEA, -α3.7, -α4.2deletion) and substitution mutation (HbCS and HbE) were genotyped using multiplex gap-PCR and PCR-RFLP, respectively. Results: In our malaria patients, 17/210 homozygous and 74/210 heterozygous -α3.7 deletion were found. Only 3/210 heterozygous -α4.2 deletion and 2/210 heterozygous--SEA deletion were detected. HbE is frequently found with 6/210 homozygote and 35/210 heterozygote. The most common thalassemia allele frequencies in Myanmar population were -α3.7 deletion (0.282), followed by HbE (0.101), HbCS (0.016), -α4.2 deletion (0.009), and --SEA deletion (0.003). Only density of P.vivax in α-thalassemia trait patients (-α3.7/-α3.7, --SEA/αα, -α3.7/-α4.2) but not in silent α-thalassemia (-α3.7/αα, -α4.2/αα, ααCS/αα) were significantly higher compared with non α-thalassemia patients (p=0.027). Density of P.falciparum significantly increased in heterozygous HbE patients (p=0.046). Conclusions: Alpha-thalassemia trait is associated with high level of P.vivax parasitemia in malaria patients in Southeast Asia.https://www.mjhid.org/index.php/mjhid/article/view/3083silent alpha-thalassemia, alpha-thalassemia trait, HbE, malaria, Southeast Asian |
| spellingShingle | Suparak Para Puncharee Mungkalasut Makamas Chanda Issarang Nuchprayoon Srivicha Krundsood Chalisa Louicharoen Cheepsunthorn AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIA Mediterranean Journal of Hematology and Infectious Diseases silent alpha-thalassemia, alpha-thalassemia trait, HbE, malaria, Southeast Asian |
| title | AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIA |
| title_full | AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIA |
| title_fullStr | AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIA |
| title_full_unstemmed | AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIA |
| title_short | AN OBSERVATIONAL STUDY OF THE EFFECT OF HEMOGLOBINOPATHY, ALPHA THALASSEMIA AND HEMOGLOBIN E ON P. VIVAX PARASITEMIA |
| title_sort | observational study of the effect of hemoglobinopathy alpha thalassemia and hemoglobin e on p vivax parasitemia |
| topic | silent alpha-thalassemia, alpha-thalassemia trait, HbE, malaria, Southeast Asian |
| url | https://www.mjhid.org/index.php/mjhid/article/view/3083 |
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