Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors
Abstract Treating metastatic brain tumors remains a significant challenge. This study introduces and applies the Patient-Derived Tumor Spheroid (PDTS) system, an ex vivo model for precision drug testing on metastatic brain tumor. The PDTS system utilizes a decellularized extracellular matrix (dECM)...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-83409-0 |
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| author | Chun-Jen Chang Kai-Wen Kan Yu-Yin Shih Yu-Han Nan Yu-Chung Juan Chih-Hsiu Tu Chen-Ting Yang Wei-Lin Hsu Der-Yang Cho Ming-You Shie Yi-Wen Chen Chun-Chung Chen |
| author_facet | Chun-Jen Chang Kai-Wen Kan Yu-Yin Shih Yu-Han Nan Yu-Chung Juan Chih-Hsiu Tu Chen-Ting Yang Wei-Lin Hsu Der-Yang Cho Ming-You Shie Yi-Wen Chen Chun-Chung Chen |
| author_sort | Chun-Jen Chang |
| collection | DOAJ |
| description | Abstract Treating metastatic brain tumors remains a significant challenge. This study introduces and applies the Patient-Derived Tumor Spheroid (PDTS) system, an ex vivo model for precision drug testing on metastatic brain tumor. The PDTS system utilizes a decellularized extracellular matrix (dECM) derived from adipose tissue, combined with the tumor cells, to form tumor spheroids. These spheroids were subsequently used to test anticancer drugs, with results compared to the clinical outcomes observed after administering these treatments to patients. To assess the validity of the data, the correlation between the drug responses observed in the PDTS model and actual patient outcomes was analyzed. Chi-square tests evaluated the significance of associations between lab predictions and clinical outcomes, using a significance threshold of p < 0.05. In preliminary data, 17 patients met the criteria for final analysis, which showed an overall 57% accuracy (p-value = 0.463), with improvements to 73% accuracy (p-value = 0.072) when patients receiving certain treatments were excluded. This PDTS offers real-time results within three weeks, simultaneous testing of multiple drugs, and the ability to culture and store tumor cells for reproducibility. Despite some limitations, further development of this model could enhance its clinical application and improve patient outcomes. |
| format | Article |
| id | doaj-art-6e76c850b04e4a89bab763e586afa6d3 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-6e76c850b04e4a89bab763e586afa6d32025-01-05T12:30:43ZengNature PortfolioScientific Reports2045-23222024-12-0114111210.1038/s41598-024-83409-0Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumorsChun-Jen Chang0Kai-Wen Kan1Yu-Yin Shih2Yu-Han Nan3Yu-Chung Juan4Chih-Hsiu Tu5Chen-Ting Yang6Wei-Lin Hsu7Der-Yang Cho8Ming-You Shie9Yi-Wen Chen10Chun-Chung Chen11Department of Neurosurgery, China Medical University Hospitalx-Dimension Center for Medical Research and Translation, China Medical University HospitalResearch and Development Center for x-Dimensional Extracellular Vesicles, China Medical University HospitalDepartment of Neurosurgery, China Medical University HospitalDepartment of Neurosurgery, China Medical University HospitalDepartment of Neurosurgery, China Medical University HospitalDepartment of Neurosurgery, China Medical University HospitalDepartment of Neurosurgery, China Medical University HospitalDepartment of Neurosurgery, China Medical University Hospitalx-Dimension Center for Medical Research and Translation, China Medical University Hospitalx-Dimension Center for Medical Research and Translation, China Medical University HospitalDepartment of Neurosurgery, China Medical University HospitalAbstract Treating metastatic brain tumors remains a significant challenge. This study introduces and applies the Patient-Derived Tumor Spheroid (PDTS) system, an ex vivo model for precision drug testing on metastatic brain tumor. The PDTS system utilizes a decellularized extracellular matrix (dECM) derived from adipose tissue, combined with the tumor cells, to form tumor spheroids. These spheroids were subsequently used to test anticancer drugs, with results compared to the clinical outcomes observed after administering these treatments to patients. To assess the validity of the data, the correlation between the drug responses observed in the PDTS model and actual patient outcomes was analyzed. Chi-square tests evaluated the significance of associations between lab predictions and clinical outcomes, using a significance threshold of p < 0.05. In preliminary data, 17 patients met the criteria for final analysis, which showed an overall 57% accuracy (p-value = 0.463), with improvements to 73% accuracy (p-value = 0.072) when patients receiving certain treatments were excluded. This PDTS offers real-time results within three weeks, simultaneous testing of multiple drugs, and the ability to culture and store tumor cells for reproducibility. Despite some limitations, further development of this model could enhance its clinical application and improve patient outcomes.https://doi.org/10.1038/s41598-024-83409-0Metastatic brain tumorPatient-derived organoidPatient-derived tumor spheroidsMicro-organospherePrecision oncology |
| spellingShingle | Chun-Jen Chang Kai-Wen Kan Yu-Yin Shih Yu-Han Nan Yu-Chung Juan Chih-Hsiu Tu Chen-Ting Yang Wei-Lin Hsu Der-Yang Cho Ming-You Shie Yi-Wen Chen Chun-Chung Chen Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors Scientific Reports Metastatic brain tumor Patient-derived organoid Patient-derived tumor spheroids Micro-organosphere Precision oncology |
| title | Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors |
| title_full | Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors |
| title_fullStr | Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors |
| title_full_unstemmed | Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors |
| title_short | Preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors |
| title_sort | preliminary study of utilizing a patient derived tumor spheroid model to augment precision therapy in metastatic brain tumors |
| topic | Metastatic brain tumor Patient-derived organoid Patient-derived tumor spheroids Micro-organosphere Precision oncology |
| url | https://doi.org/10.1038/s41598-024-83409-0 |
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