Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharides
As has been confirmed in our previous study, red onion is a promising source of inulin-fructan, inulin neoseries fructan, and their fructooligosaccharides (FOSs). Short-chain FOSs (SCFOSs) are potential prebiotics as they provide many health promoting benefits. However, short-chain inulin neoseries...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324003909 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846121240091164672 |
|---|---|
| author | Jirat Wongsanittayarak Nalapat Leangnim Kridsada Unban Chartchai Khanongnuch Saisamorn Lumyong Pairote Wongputtisin Apinun Kanpiengjai |
| author_facet | Jirat Wongsanittayarak Nalapat Leangnim Kridsada Unban Chartchai Khanongnuch Saisamorn Lumyong Pairote Wongputtisin Apinun Kanpiengjai |
| author_sort | Jirat Wongsanittayarak |
| collection | DOAJ |
| description | As has been confirmed in our previous study, red onion is a promising source of inulin-fructan, inulin neoseries fructan, and their fructooligosaccharides (FOSs). Short-chain FOSs (SCFOSs) are potential prebiotics as they provide many health promoting benefits. However, short-chain inulin neoseries oligosaccharides isolated from plants have scarcely been studied. After prebiotic production, a purification step is essential to produce a functional prebiotic. The aim of this study was to develop new processes of integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of SCFOSs. Endo-inulinase is a key enzyme for hydrolysis of crude red onion extract to produce SCFOSs, while Candida orthopsilosis FLA44.2 was employed for purification of the produced SCFOSs. Three different strategies, including two-step (TSP), simultaneous (SP), and semi-simultaneous (SSP) production processes, were designed and investigated as potentially simple, cost-effective, and timesaving production processes. The SP and SSP processes met the objective criteria by exhibiting desirable yields of SCFOSs and the optimal purity of SCFOSs when compared with the TSP process. Notably, the SP process was highlighted as it was simpler than the SSP process. Under optimal conditions (0.4 U of endo-inulinase/g total fructans, 5 % v/v of yeast inoculum and reaction time of 72 h), the SP process conducted in 1-dm3 flasks produced SCFOSs of 87 g/dm3 with neo-GF2 as the main constituent (42 g/dm3) and a purity of 100 %. The ratio of total SCFOSs to total fructans was 0.93 indicating that fructans of the red onion extract have been transformed to SCFOSs. |
| format | Article |
| id | doaj-art-591b433b559f404aaf4916abe2b04736 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-591b433b559f404aaf4916abe2b047362024-12-16T05:37:29ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-0118101353Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharidesJirat Wongsanittayarak0Nalapat Leangnim1Kridsada Unban2Chartchai Khanongnuch3Saisamorn Lumyong4Pairote Wongputtisin5Apinun Kanpiengjai6Program in Biotechnology, Multidisciplinary and Interdisciplinary School, Chiang Mai University, Chiang Mai, 50200, Thailand; Division of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, ThailandDivision of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Office of Research Administration, Chiang Mai University, Chiang Mai, 50200, ThailandDivision of Food Science and Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Academy of Science, The Royal Society of Thailand, Bangkok, 10300, ThailandProgram in Biotechnology, Faculty of Science, Maejo University, Chiang Mai, 50290, ThailandDivision of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Corresponding author.As has been confirmed in our previous study, red onion is a promising source of inulin-fructan, inulin neoseries fructan, and their fructooligosaccharides (FOSs). Short-chain FOSs (SCFOSs) are potential prebiotics as they provide many health promoting benefits. However, short-chain inulin neoseries oligosaccharides isolated from plants have scarcely been studied. After prebiotic production, a purification step is essential to produce a functional prebiotic. The aim of this study was to develop new processes of integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of SCFOSs. Endo-inulinase is a key enzyme for hydrolysis of crude red onion extract to produce SCFOSs, while Candida orthopsilosis FLA44.2 was employed for purification of the produced SCFOSs. Three different strategies, including two-step (TSP), simultaneous (SP), and semi-simultaneous (SSP) production processes, were designed and investigated as potentially simple, cost-effective, and timesaving production processes. The SP and SSP processes met the objective criteria by exhibiting desirable yields of SCFOSs and the optimal purity of SCFOSs when compared with the TSP process. Notably, the SP process was highlighted as it was simpler than the SSP process. Under optimal conditions (0.4 U of endo-inulinase/g total fructans, 5 % v/v of yeast inoculum and reaction time of 72 h), the SP process conducted in 1-dm3 flasks produced SCFOSs of 87 g/dm3 with neo-GF2 as the main constituent (42 g/dm3) and a purity of 100 %. The ratio of total SCFOSs to total fructans was 0.93 indicating that fructans of the red onion extract have been transformed to SCFOSs.http://www.sciencedirect.com/science/article/pii/S2666154324003909Red onionFructooligosaccharidesNeofructooliogsaccharidesNeokestoseEndo-inulinaseYeast treatment |
| spellingShingle | Jirat Wongsanittayarak Nalapat Leangnim Kridsada Unban Chartchai Khanongnuch Saisamorn Lumyong Pairote Wongputtisin Apinun Kanpiengjai Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharides Journal of Agriculture and Food Research Red onion Fructooligosaccharides Neofructooliogsaccharides Neokestose Endo-inulinase Yeast treatment |
| title | Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharides |
| title_full | Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharides |
| title_fullStr | Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharides |
| title_full_unstemmed | Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharides |
| title_short | Integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short-chain inulin and inulin neoseries oligosaccharides |
| title_sort | integrated enzymatic hydrolysis of crude red onion extract and yeast treatment for production and purification of short chain inulin and inulin neoseries oligosaccharides |
| topic | Red onion Fructooligosaccharides Neofructooliogsaccharides Neokestose Endo-inulinase Yeast treatment |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324003909 |
| work_keys_str_mv | AT jiratwongsanittayarak integratedenzymatichydrolysisofcruderedonionextractandyeasttreatmentforproductionandpurificationofshortchaininulinandinulinneoseriesoligosaccharides AT nalapatleangnim integratedenzymatichydrolysisofcruderedonionextractandyeasttreatmentforproductionandpurificationofshortchaininulinandinulinneoseriesoligosaccharides AT kridsadaunban integratedenzymatichydrolysisofcruderedonionextractandyeasttreatmentforproductionandpurificationofshortchaininulinandinulinneoseriesoligosaccharides AT chartchaikhanongnuch integratedenzymatichydrolysisofcruderedonionextractandyeasttreatmentforproductionandpurificationofshortchaininulinandinulinneoseriesoligosaccharides AT saisamornlumyong integratedenzymatichydrolysisofcruderedonionextractandyeasttreatmentforproductionandpurificationofshortchaininulinandinulinneoseriesoligosaccharides AT pairotewongputtisin integratedenzymatichydrolysisofcruderedonionextractandyeasttreatmentforproductionandpurificationofshortchaininulinandinulinneoseriesoligosaccharides AT apinunkanpiengjai integratedenzymatichydrolysisofcruderedonionextractandyeasttreatmentforproductionandpurificationofshortchaininulinandinulinneoseriesoligosaccharides |