Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae
Introduction: The aim of this work was to study the ability of ciprofloxacin to restrict the development of resistant mutants of Escherichia coli and Klebsiella pneumoniae through determination of the Mutant Prevention Concentration (MPC). Methodology: We studied 140 strains of E. coli and 86 strai...
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The Journal of Infection in Developing Countries
2014-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/3164 |
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| author | Ziad Daoud Elie Salem Sokhn Eid Azar Khalil Masri Shira Doron |
| author_facet | Ziad Daoud Elie Salem Sokhn Eid Azar Khalil Masri Shira Doron |
| author_sort | Ziad Daoud |
| collection | DOAJ |
| description | Introduction: The aim of this work was to study the ability of ciprofloxacin to restrict the development of resistant mutants of Escherichia coli and Klebsiella pneumoniae through determination of the Mutant Prevention Concentration (MPC).
Methodology: We studied 140 strains of E. coli and 86 strains of K. pneumoniae with different profiles of sensitivity to fluoroquinolones and extended spectrum beta lactamase (ESBL) production. The MPCs were determined using an inoculum of 1010 CFU/ml in Mueller-Hinton agar plates with serial concentrations of ciprofloxacin.
Results: Ciprofloxacin-susceptible ESBL-producing strains showed a higher MPC for ciprofloxacin (P <0.001) than ciprofloxacin-susceptible non ESBL-producing strains, while ciprofloxacin-resistant ESBL-producing and non ESBL-producing strains did not significantly differ. The presence of qnr variants was associated with elevated MPCs. This was observed for both tested organisms.
Conclusions: Our study helps to explain the frequent finding of resistance to fluoroquinolones in ESBL-producing strains. Consequently, the use of concentrations of ciprofloxacin higher than the MIC in order to prevent the recovery and growth of resistant mutants is recommended.
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| format | Article |
| id | doaj-art-51a5fc62a17d48779a9b0b51f58c64f0 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-51a5fc62a17d48779a9b0b51f58c64f02025-08-20T02:57:12ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-02-0180210.3855/jidc.3164Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniaeZiad Daoud0Elie Salem Sokhn1Eid Azar2Khalil Masri3Shira Doron4University of Balamand, Tripoli, LebanonUniversity of Surrey, Guildford, United KingdomUniversity of Balamand, Tripoli, LebanonCentre Hospitalier du Nord, Zgharta, LebanonTufts Medical Center, Boston, MA,United StatesIntroduction: The aim of this work was to study the ability of ciprofloxacin to restrict the development of resistant mutants of Escherichia coli and Klebsiella pneumoniae through determination of the Mutant Prevention Concentration (MPC). Methodology: We studied 140 strains of E. coli and 86 strains of K. pneumoniae with different profiles of sensitivity to fluoroquinolones and extended spectrum beta lactamase (ESBL) production. The MPCs were determined using an inoculum of 1010 CFU/ml in Mueller-Hinton agar plates with serial concentrations of ciprofloxacin. Results: Ciprofloxacin-susceptible ESBL-producing strains showed a higher MPC for ciprofloxacin (P <0.001) than ciprofloxacin-susceptible non ESBL-producing strains, while ciprofloxacin-resistant ESBL-producing and non ESBL-producing strains did not significantly differ. The presence of qnr variants was associated with elevated MPCs. This was observed for both tested organisms. Conclusions: Our study helps to explain the frequent finding of resistance to fluoroquinolones in ESBL-producing strains. Consequently, the use of concentrations of ciprofloxacin higher than the MIC in order to prevent the recovery and growth of resistant mutants is recommended. https://jidc.org/index.php/journal/article/view/3164mutant prevention concentrationESBLciprofloxacinEscherichia coliKlebsiella pneumoniae |
| spellingShingle | Ziad Daoud Elie Salem Sokhn Eid Azar Khalil Masri Shira Doron Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae Journal of Infection in Developing Countries mutant prevention concentration ESBL ciprofloxacin Escherichia coli Klebsiella pneumoniae |
| title | Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae |
| title_full | Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae |
| title_fullStr | Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae |
| title_full_unstemmed | Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae |
| title_short | Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae |
| title_sort | mutant prevention concentrations of ciprofloxacin against urinary isolates of escherichia coli and klebsiella pneumoniae |
| topic | mutant prevention concentration ESBL ciprofloxacin Escherichia coli Klebsiella pneumoniae |
| url | https://jidc.org/index.php/journal/article/view/3164 |
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