Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA
Abstract MicroRNAs(miRNAs) are promising biomarkers for early esophageal squamous cell carcinoma (ESCC) detection and prognostic prediction. This study aimed to explore the potential biomarkers and molecular pathogenesis in the early diagnosis of ESCC. Firstly, 48 differentially expressed miRNAs (DE...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-76321-0 |
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| author | Ping Yuan Xiaoyan Gao Mingjun Xu Liangyu Qiu Zijun Xiong Jun Shen Huanhuan Xing Ruofan Yang Liang Zhao Xi Liu Jiaowei Gu Wenting Liu |
| author_facet | Ping Yuan Xiaoyan Gao Mingjun Xu Liangyu Qiu Zijun Xiong Jun Shen Huanhuan Xing Ruofan Yang Liang Zhao Xi Liu Jiaowei Gu Wenting Liu |
| author_sort | Ping Yuan |
| collection | DOAJ |
| description | Abstract MicroRNAs(miRNAs) are promising biomarkers for early esophageal squamous cell carcinoma (ESCC) detection and prognostic prediction. This study aimed to explore the potential biomarkers and molecular pathogenesis in the early diagnosis of ESCC. Firstly, 48 differentially expressed miRNAs (DEMs) and 1319 differentially expressed genes (DEGs) were identified between 94 ESCC tissues and 13 normal esophageal tissues in TCGA. From miRNA–mRNA regulatory network, there are 6558 target genes of the 48 DEMs, where 400 target genes are also among 1319 DEGs. Then, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicate that the 400 DEGs significantly enriched in cell cycle, proteoglycans in cancer, p53 signaling pathway, protein digestion and absorption, transcriptional dysregulation in cancer, and oocyte meiosis. And there are 66 DEGs among these six biological pathways, which we called GO-DEGs. From miRNA–mRNA regulatory network, 32 DEMs regulated the 66 GO-DEGs, where 22 DEMs were verified by different types of experiments in ESCC tissues, cells, or serum from the literature. For the other novel 10 DEMs, single-factor Cox regression analysis show that only hsa-miR-34b-3p showed no significant correlation with the overall survival of ESCC patients. Finally, we obtained the novel 9 ESCC-related DEMs, where three are down-regulated, and six are up-regulated. We analyzed the expression trends of target genes for five miRNAs and identified three significantly different miRNAs (hsa-miR-205-3p, hsa-miR-452-3p, and hsa-miR-6499-3p) confirmed by qPCR. Moreover, the stage-specific miRNAs were also suggested. These three qPCR validated miRNAs are also specific to the early stages of ESCC: hsa-miR-452-3p is specific to Stage I, II and III; hsa-miR-205-3p is specific in Stage II and III; and hsa-miR-6499-3p is Stage II specific. They might be the potential biomarkers for ESCC stage diagnosis. This study identified three novel miRNA markers potentially related to the diagnosis of ESCC and participated in the occurrence and development of ESCC through cell cycle, proteoglycans in cancer, p53 signaling pathway, protein digestion and absorption, transcriptional dysregulation in cancer, and signaling pathway for oocyte meiosis. |
| format | Article |
| id | doaj-art-49fe7272651f482f8c3d5e55d222abeb |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-49fe7272651f482f8c3d5e55d222abeb2024-11-10T12:27:00ZengNature PortfolioScientific Reports2045-23222024-11-0114111510.1038/s41598-024-76321-0Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGAPing Yuan0Xiaoyan Gao1Mingjun Xu2Liangyu Qiu3Zijun Xiong4Jun Shen5Huanhuan Xing6Ruofan Yang7Liang Zhao8Xi Liu9Jiaowei Gu10Wenting Liu11Healthcare Big Data Center, School of Public Health, Hubei University of MedicineRenmin Hospital, Hubei University of MedicineTaihe Hospital, Hubei University of MedicineTaihe Hospital, Hubei University of MedicineHealthcare Big Data Center, School of Public Health, Hubei University of MedicineRenmin Hospital, Hubei University of MedicineHealthcare Big Data Center, School of Public Health, Hubei University of MedicineHealthcare Big Data Center, School of Public Health, Hubei University of MedicinePrecision Medicine Research Center, Taihe Hospital, Hubei University of MedicineHealthcare Big Data Center, School of Public Health, Hubei University of MedicineHubei Provincial Clinical Research Center for Umbilical Cord Blood Hematopoietic Stem Cells, Taihe Hospital, Hubei University of MedicineHealthcare Big Data Center, School of Public Health, Hubei University of MedicineAbstract MicroRNAs(miRNAs) are promising biomarkers for early esophageal squamous cell carcinoma (ESCC) detection and prognostic prediction. This study aimed to explore the potential biomarkers and molecular pathogenesis in the early diagnosis of ESCC. Firstly, 48 differentially expressed miRNAs (DEMs) and 1319 differentially expressed genes (DEGs) were identified between 94 ESCC tissues and 13 normal esophageal tissues in TCGA. From miRNA–mRNA regulatory network, there are 6558 target genes of the 48 DEMs, where 400 target genes are also among 1319 DEGs. Then, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicate that the 400 DEGs significantly enriched in cell cycle, proteoglycans in cancer, p53 signaling pathway, protein digestion and absorption, transcriptional dysregulation in cancer, and oocyte meiosis. And there are 66 DEGs among these six biological pathways, which we called GO-DEGs. From miRNA–mRNA regulatory network, 32 DEMs regulated the 66 GO-DEGs, where 22 DEMs were verified by different types of experiments in ESCC tissues, cells, or serum from the literature. For the other novel 10 DEMs, single-factor Cox regression analysis show that only hsa-miR-34b-3p showed no significant correlation with the overall survival of ESCC patients. Finally, we obtained the novel 9 ESCC-related DEMs, where three are down-regulated, and six are up-regulated. We analyzed the expression trends of target genes for five miRNAs and identified three significantly different miRNAs (hsa-miR-205-3p, hsa-miR-452-3p, and hsa-miR-6499-3p) confirmed by qPCR. Moreover, the stage-specific miRNAs were also suggested. These three qPCR validated miRNAs are also specific to the early stages of ESCC: hsa-miR-452-3p is specific to Stage I, II and III; hsa-miR-205-3p is specific in Stage II and III; and hsa-miR-6499-3p is Stage II specific. They might be the potential biomarkers for ESCC stage diagnosis. This study identified three novel miRNA markers potentially related to the diagnosis of ESCC and participated in the occurrence and development of ESCC through cell cycle, proteoglycans in cancer, p53 signaling pathway, protein digestion and absorption, transcriptional dysregulation in cancer, and signaling pathway for oocyte meiosis.https://doi.org/10.1038/s41598-024-76321-0Esophageal squamous cell carcinomamiRNAmRNABiomarkers |
| spellingShingle | Ping Yuan Xiaoyan Gao Mingjun Xu Liangyu Qiu Zijun Xiong Jun Shen Huanhuan Xing Ruofan Yang Liang Zhao Xi Liu Jiaowei Gu Wenting Liu Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA Scientific Reports Esophageal squamous cell carcinoma miRNA mRNA Biomarkers |
| title | Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA |
| title_full | Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA |
| title_fullStr | Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA |
| title_full_unstemmed | Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA |
| title_short | Novel miRNA markers and their mechanism of esophageal squamous cell carcinoma (ESCC) based on TCGA |
| title_sort | novel mirna markers and their mechanism of esophageal squamous cell carcinoma escc based on tcga |
| topic | Esophageal squamous cell carcinoma miRNA mRNA Biomarkers |
| url | https://doi.org/10.1038/s41598-024-76321-0 |
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