The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells
Tongue squamous cell carcinoma is the most common malignant tumor in oral and maxillofacial regions. Recent research has found that artemether can inhibit growth and induce apoptosis of cancer cells, although the mechanism is not clear. The present study aimed to explore the correlation between the...
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12711 |
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| author | Jianhua Wu Lei Li Yiting Wang Xiaobin Ren Ken Lin Yongwen He |
| author_facet | Jianhua Wu Lei Li Yiting Wang Xiaobin Ren Ken Lin Yongwen He |
| author_sort | Jianhua Wu |
| collection | DOAJ |
| description | Tongue squamous cell carcinoma is the most common malignant tumor in oral and maxillofacial regions. Recent research has found that artemether can inhibit growth and induce apoptosis of cancer cells, although the mechanism is not clear. The present study aimed to explore the correlation between the HSP90/Akt pathway and artemether‐induced apoptosis of Cal27 cells. A cell counting kit‐8 and flow cytometry were used to detect the proliferation and apoptosis of Cal27 cells, respectively, mRNA expression was examined by quantitative RT‐PCR, and protein expression was detected by western blotting. Our data revealed that artemether can inhibit growth and induce apoptosis of Cal27 cells. As the artemether concentration was increased, we observed downregulation of the expression of HSP90, p‐Akt and p‐mTOR in Cal27 cells, whereas the expression of Akt was not significantly changed. We also observed a time‐dependent decrease in the expression of HSP90, p‐Akt and p‐mTOR during exposure to 0.1 mg·mL−1 artemether. In conclusion, the HSP90/Akt pathway may be involved in artemether‐induced apoptosis of Cal27 cells. |
| format | Article |
| id | doaj-art-46705b0615f44601ac2a806f5af80ff7 |
| institution | Kabale University |
| issn | 2211-5463 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-46705b0615f44601ac2a806f5af80ff72024-12-06T11:26:04ZengWileyFEBS Open Bio2211-54632019-10-019101726173310.1002/2211-5463.12711The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cellsJianhua Wu0Lei Li1Yiting Wang2Xiaobin Ren3Ken Lin4Yongwen He5Department of Periodontology The Affiliated Stomatological Hospital of Kunming Medical University Kunming ChinaDepartment of Head and Neck Surgery The Third Affiliated Hospital of Kunming Medical University Kunming ChinaDepartment of Oral and Maxillofacial Surgery The Affiliated Stomatological Hospital of Kunming Medical University Kunming ChinaDepartment of Periodontology The Affiliated Stomatological Hospital of Kunming Medical University Kunming ChinaDepartment of Otolaryngology, Head and Neck Surgery Kunming Children's Hospital Kunming ChinaDepartment of Dental Research The Affiliated Stomatological Hospital of Kunming Medical University Kunming ChinaTongue squamous cell carcinoma is the most common malignant tumor in oral and maxillofacial regions. Recent research has found that artemether can inhibit growth and induce apoptosis of cancer cells, although the mechanism is not clear. The present study aimed to explore the correlation between the HSP90/Akt pathway and artemether‐induced apoptosis of Cal27 cells. A cell counting kit‐8 and flow cytometry were used to detect the proliferation and apoptosis of Cal27 cells, respectively, mRNA expression was examined by quantitative RT‐PCR, and protein expression was detected by western blotting. Our data revealed that artemether can inhibit growth and induce apoptosis of Cal27 cells. As the artemether concentration was increased, we observed downregulation of the expression of HSP90, p‐Akt and p‐mTOR in Cal27 cells, whereas the expression of Akt was not significantly changed. We also observed a time‐dependent decrease in the expression of HSP90, p‐Akt and p‐mTOR during exposure to 0.1 mg·mL−1 artemether. In conclusion, the HSP90/Akt pathway may be involved in artemether‐induced apoptosis of Cal27 cells.https://doi.org/10.1002/2211-5463.12711AktapoptosisartemetherHSP90tongue squamous cell carcinoma |
| spellingShingle | Jianhua Wu Lei Li Yiting Wang Xiaobin Ren Ken Lin Yongwen He The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells FEBS Open Bio Akt apoptosis artemether HSP90 tongue squamous cell carcinoma |
| title | The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells |
| title_full | The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells |
| title_fullStr | The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells |
| title_full_unstemmed | The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells |
| title_short | The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells |
| title_sort | hsp90 akt pathway may mediate artemether induced apoptosis of cal27 cells |
| topic | Akt apoptosis artemether HSP90 tongue squamous cell carcinoma |
| url | https://doi.org/10.1002/2211-5463.12711 |
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