Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro
Relevance. Currently, food products that include prebiotics, in particular, inulin, are particularly popular. Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assim...
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Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center"
2021-11-01
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| Series: | Овощи России |
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| Online Access: | https://www.vegetables.su/jour/article/view/1852 |
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| author | E. A. Kalashnikova R. N. Kirakosyan A. V. Gushchin K. G. Abubakarov N. N. Sleptsov S. K. Temirbekova A. P. Glinushkin O. V. Meleshina N. Ya. Rebouh M. M. Tareeva |
| author_facet | E. A. Kalashnikova R. N. Kirakosyan A. V. Gushchin K. G. Abubakarov N. N. Sleptsov S. K. Temirbekova A. P. Glinushkin O. V. Meleshina N. Ya. Rebouh M. M. Tareeva |
| author_sort | E. A. Kalashnikova |
| collection | DOAJ |
| description | Relevance. Currently, food products that include prebiotics, in particular, inulin, are particularly popular. Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assimilation of useful trace elements necessary for human life. Inulin is contained in plants such as jerusalem artichoke, chicory, as well as in sweet potatoes, the popularity of which is increasing every year. However, sweet potato plants are afraid of cold and frost-resistant. Therefore, the creation of new varieties and hybrids that are resistant to low temperatures is an urgent problem. Cellular biotechnology is aimed at solving this problem using methods of clonal microreproduction, cell selection, somatic hybridization, etc. For rapid reproduction and obtaining high-quality planting material, biotechnology methods are used, in particular, clonal micro-propagation. However, in this technology there are difficulties associated with poor adaptation of microclones to ex vitro conditions. This fact introduces an additional requirement for the selection of optimal rooting modes in vitro and ex vitro adaptation of microclones.Material and methodology. The aim of the work was to study the influence of cultivation conditions on in vitro rooting and ex vitro adaptation of I. batatas (L.) microclones. The object of the study was sweet potato microgears propagated in vitro. I. batatas micro-gears were cultured in vitro on a Murashige-Skug medium, differing by the type of auxins. The influence of red (R) and far red (FR) light on the shoots rooting in vitro and the adaptation of microclones ex vitro was studied.Results. It has been experimentally established that the cultivation of micro-gears on a medium containing indolyl butyric acid at a concentration of 0.5-1 mg/l and under conditions of illumination by LED lamps of red and far red light in equal amounts leads to the production of microclones with a well-developed root system and vegetative biomass. The use of an aeroponic installation at the last stage of clonal micro-propagation makes it possible to obtain high-quality planting material that can adapt well to open ground conditions. |
| format | Article |
| id | doaj-art-427b7f98b3e34cec8f33d2d310ad44cf |
| institution | Kabale University |
| issn | 2072-9146 2618-7132 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center" |
| record_format | Article |
| series | Овощи России |
| spelling | doaj-art-427b7f98b3e34cec8f33d2d310ad44cf2025-08-20T03:43:21ZengFederal State Budgetary Scientific Institution "Federal Scientific Vegetable Center"Овощи России2072-91462618-71322021-11-0106222910.18619/2072-9146-2021-6-22-291313Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitroE. A. Kalashnikova0R. N. Kirakosyan1A. V. Gushchin2K. G. Abubakarov3N. N. Sleptsov4S. K. Temirbekova5A. P. Glinushkin6O. V. Meleshina7N. Ya. Rebouh8M. M. Tareeva9Russian State Agrarian University – Moscow Timiryazev Agricultural AcademyRussian State Agrarian University – Moscow Timiryazev Agricultural AcademyRussian State Agrarian University – Moscow Timiryazev Agricultural AcademyRussian State Agrarian University – Moscow Timiryazev Agricultural AcademyRussian State Agrarian University – Moscow Timiryazev Agricultural AcademyFederal State Budgetary Scientific Institution All-Russian Res earch Institute of hytopathology B. VyazemyFederal State Budgetary Scientific Institution All-Russian Res earch Institute of hytopathology B. VyazemyFederal State Budgetary Scientific Institution All-Russian Res earch Institute of hytopathology B. VyazemyPeoples Friendship University of Russia (RUDN University)Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC)Relevance. Currently, food products that include prebiotics, in particular, inulin, are particularly popular. Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assimilation of useful trace elements necessary for human life. Inulin is contained in plants such as jerusalem artichoke, chicory, as well as in sweet potatoes, the popularity of which is increasing every year. However, sweet potato plants are afraid of cold and frost-resistant. Therefore, the creation of new varieties and hybrids that are resistant to low temperatures is an urgent problem. Cellular biotechnology is aimed at solving this problem using methods of clonal microreproduction, cell selection, somatic hybridization, etc. For rapid reproduction and obtaining high-quality planting material, biotechnology methods are used, in particular, clonal micro-propagation. However, in this technology there are difficulties associated with poor adaptation of microclones to ex vitro conditions. This fact introduces an additional requirement for the selection of optimal rooting modes in vitro and ex vitro adaptation of microclones.Material and methodology. The aim of the work was to study the influence of cultivation conditions on in vitro rooting and ex vitro adaptation of I. batatas (L.) microclones. The object of the study was sweet potato microgears propagated in vitro. I. batatas micro-gears were cultured in vitro on a Murashige-Skug medium, differing by the type of auxins. The influence of red (R) and far red (FR) light on the shoots rooting in vitro and the adaptation of microclones ex vitro was studied.Results. It has been experimentally established that the cultivation of micro-gears on a medium containing indolyl butyric acid at a concentration of 0.5-1 mg/l and under conditions of illumination by LED lamps of red and far red light in equal amounts leads to the production of microclones with a well-developed root system and vegetative biomass. The use of an aeroponic installation at the last stage of clonal micro-propagation makes it possible to obtain high-quality planting material that can adapt well to open ground conditions.https://www.vegetables.su/jour/article/view/1852batatgrowth regulatorsspectral composition of lightledsmicroclonesaeroponicsin vitroex vitro |
| spellingShingle | E. A. Kalashnikova R. N. Kirakosyan A. V. Gushchin K. G. Abubakarov N. N. Sleptsov S. K. Temirbekova A. P. Glinushkin O. V. Meleshina N. Ya. Rebouh M. M. Tareeva Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro Овощи России batat growth regulators spectral composition of light leds microclones aeroponics in vitro ex vitro |
| title | Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro |
| title_full | Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro |
| title_fullStr | Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro |
| title_full_unstemmed | Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro |
| title_short | Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro |
| title_sort | ipomoea batatas l lam cultivation in the conditions of light culture in vitro and ex vitro |
| topic | batat growth regulators spectral composition of light leds microclones aeroponics in vitro ex vitro |
| url | https://www.vegetables.su/jour/article/view/1852 |
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