COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTS

Objective: The human chorionic gonadotropin (hCG) stimulation test is widely used to assess testicular steroidogenesis. This test evaluates Leydig cell function and helps to evaluate testosterone synthesis in testicular enzyme defects, hypogonadism, 46,XY sexual development disorders (DSD), and micr...

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Main Authors: Aslı Derya Kardelen Al, Esin Karakılıç Özturan, Leyla Asgarova, Ayşe Pınar Öztürk, Melek Yıldız, Şükran Poyrazoğlu, Firdevs Baş, Fatma Feyza Darendeliler
Format: Article
Language:English
Published: Istanbul University Press 2022-03-01
Series:İstanbul Tıp Fakültesi Dergisi
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Online Access:https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/D9C36C516E664F429D917A1BDE3A4BB2
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author Aslı Derya Kardelen Al
Esin Karakılıç Özturan
Leyla Asgarova
Ayşe Pınar Öztürk
Melek Yıldız
Şükran Poyrazoğlu
Firdevs Baş
Fatma Feyza Darendeliler
author_facet Aslı Derya Kardelen Al
Esin Karakılıç Özturan
Leyla Asgarova
Ayşe Pınar Öztürk
Melek Yıldız
Şükran Poyrazoğlu
Firdevs Baş
Fatma Feyza Darendeliler
author_sort Aslı Derya Kardelen Al
collection DOAJ
description Objective: The human chorionic gonadotropin (hCG) stimulation test is widely used to assess testicular steroidogenesis. This test evaluates Leydig cell function and helps to evaluate testosterone synthesis in testicular enzyme defects, hypogonadism, 46,XY sexual development disorders (DSD), and micropenis. This study will compare testosterone responses to a single dose of 5000 IU/m2 and three consecutive days of 1500 IU/m2 hCG regimens. Materials and Methods: The study evaluated 18 patients who were admitted with micropenis, hypospadias, or undescended testis (Group 1) and 18 patients with a molecular diagnosis of androgen receptor insensitivity (56%) or 5α reductase deficiency (44%) (Group 2). The median (interquartile range; IQR) age of the patients were 1.4 (0.8-4.2) years for Group 1 and 0.7 (0.3-0.8) years for Group 2. Baseline testosterone levels were checked and after a single dose of hCG 5000 IU/m2 injection into the first group, testosterone levels were observed on day one and day four. In the second group, after the injection of hCG 1500 IU/m2 for three days, testosterone levels were checked on the fourth day. Results: There was no significant difference in height, weight, BMI SDS, and baseline testosterone levels between groups. After hCG, stimulation testosterone responses did not vary among the groups. Testosterone levels were increased 40-fold and 40.1- fold on day four compared to the baseline in Group 1 and Group 2. In Group 1, testosterone response was significantly higher on day four compared to day one (p=0.001). Conclusions: The hCG tests performed with a single dose or multidoses showed that there was no difference between testosterone responses and these two tests were applicable the 5000 IU/m2 hCG test requires fewer injections and is easy to administer, this test may be preferred to an evaluation of the Leydig cell function. For an adequate testosterone response, the researchers suggest measuring testosterone levels on the fourth day after the single dose of 5000 IU/m2 .
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series İstanbul Tıp Fakültesi Dergisi
spelling doaj-art-34cb4c8d39fc4573b46a91e309db856c2025-08-20T02:15:19ZengIstanbul University Pressİstanbul Tıp Fakültesi Dergisi1305-64412022-03-0185222322710.26650/IUITFD.949141123456COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTSAslı Derya Kardelen Al0https://orcid.org/0000-0003-0594-8741Esin Karakılıç Özturan1https://orcid.org/0000-0002-8842-1752Leyla Asgarova2https://orcid.org/0000-0001-9339-0500Ayşe Pınar Öztürk3https://orcid.org/0000-0003-3466-2857Melek Yıldız4https://orcid.org/0000-0002-6603-2983Şükran Poyrazoğlu5https://orcid.org/0000-0001-6806-9678Firdevs Baş6https://orcid.org/0000-0001-9689-4464Fatma Feyza Darendeliler7https://orcid.org/0000-0003-4786-0780İstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, TürkiyeObjective: The human chorionic gonadotropin (hCG) stimulation test is widely used to assess testicular steroidogenesis. This test evaluates Leydig cell function and helps to evaluate testosterone synthesis in testicular enzyme defects, hypogonadism, 46,XY sexual development disorders (DSD), and micropenis. This study will compare testosterone responses to a single dose of 5000 IU/m2 and three consecutive days of 1500 IU/m2 hCG regimens. Materials and Methods: The study evaluated 18 patients who were admitted with micropenis, hypospadias, or undescended testis (Group 1) and 18 patients with a molecular diagnosis of androgen receptor insensitivity (56%) or 5α reductase deficiency (44%) (Group 2). The median (interquartile range; IQR) age of the patients were 1.4 (0.8-4.2) years for Group 1 and 0.7 (0.3-0.8) years for Group 2. Baseline testosterone levels were checked and after a single dose of hCG 5000 IU/m2 injection into the first group, testosterone levels were observed on day one and day four. In the second group, after the injection of hCG 1500 IU/m2 for three days, testosterone levels were checked on the fourth day. Results: There was no significant difference in height, weight, BMI SDS, and baseline testosterone levels between groups. After hCG, stimulation testosterone responses did not vary among the groups. Testosterone levels were increased 40-fold and 40.1- fold on day four compared to the baseline in Group 1 and Group 2. In Group 1, testosterone response was significantly higher on day four compared to day one (p=0.001). Conclusions: The hCG tests performed with a single dose or multidoses showed that there was no difference between testosterone responses and these two tests were applicable the 5000 IU/m2 hCG test requires fewer injections and is easy to administer, this test may be preferred to an evaluation of the Leydig cell function. For an adequate testosterone response, the researchers suggest measuring testosterone levels on the fourth day after the single dose of 5000 IU/m2 .https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/D9C36C516E664F429D917A1BDE3A4BB2disorders of sex developmenthcg stimulation testtestosterone46xy
spellingShingle Aslı Derya Kardelen Al
Esin Karakılıç Özturan
Leyla Asgarova
Ayşe Pınar Öztürk
Melek Yıldız
Şükran Poyrazoğlu
Firdevs Baş
Fatma Feyza Darendeliler
COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTS
İstanbul Tıp Fakültesi Dergisi
disorders of sex development
hcg stimulation test
testosterone
46
xy
title COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTS
title_full COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTS
title_fullStr COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTS
title_full_unstemmed COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTS
title_short COMPARISON OF SINGLE DOSE AND MULTI-DOSE hCG STIMULATION TESTS
title_sort comparison of single dose and multi dose hcg stimulation tests
topic disorders of sex development
hcg stimulation test
testosterone
46
xy
url https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/D9C36C516E664F429D917A1BDE3A4BB2
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AT aysepınarozturk comparisonofsingledoseandmultidosehcgstimulationtests
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