Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii
Abstract Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent bacterial threat to public health, with only a few treatment options and a > 50% fatality rate. Although several resistance mechanisms are understood, it is still impossible to predict which mutations are most likely to occ...
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| Format: | Article |
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-81501-z |
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| author | Bogdan M. Benin Trae Hillyer Noah Aguirre Yuk Yin Sham Belinda Willard Woo Shik Shin |
| author_facet | Bogdan M. Benin Trae Hillyer Noah Aguirre Yuk Yin Sham Belinda Willard Woo Shik Shin |
| author_sort | Bogdan M. Benin |
| collection | DOAJ |
| description | Abstract Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent bacterial threat to public health, with only a few treatment options and a > 50% fatality rate. Although several resistance mechanisms are understood, it is still impossible to predict which mutations are most likely to occur. Here, we demonstrate that independent samples of Ab, exposed to different carbapenems with escalating concentrations, show concentration- and carbapenem-dependent trends in β-lactamase-isoform expression. This result, based on the isoforms identified through label-free-quantification LC-MS/MS measurements of cell-free, gel-separated β-lactamases, suggests that the appearance of antibiotic resistance may be have some predictability based on structural factors. Additionally, the identified minor isoforms also have high sequence similarity to major expressed isoforms, indicating a potential path over which resistance occurred in independent samples. Antibiotic resistance maybe therefore somewhat predictable based on specific structural properties and further investigation may lead to new strategies for mitigating antibiotic resistance. |
| format | Article |
| id | doaj-art-29fa4c864f8745a2bd61fbd8a43aabb3 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-29fa4c864f8745a2bd61fbd8a43aabb32024-12-29T12:16:44ZengNature PortfolioScientific Reports2045-23222024-12-011411910.1038/s41598-024-81501-zCarbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumanniiBogdan M. Benin0Trae Hillyer1Noah Aguirre2Yuk Yin Sham3Belinda Willard4Woo Shik Shin5Department of Pharmaceutical Sciences, Northeast Ohio Medical UniversityDepartment of Pharmaceutical Sciences, Northeast Ohio Medical UniversityDepartment of Pharmaceutical Sciences, Northeast Ohio Medical UniversityDepartment of Integrative Biology and Physiology, University of MinnesotaProteomics and Metabolomics Core, Lerner Research Institute, Cleveland ClinicDepartment of Pharmaceutical Sciences, Northeast Ohio Medical UniversityAbstract Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent bacterial threat to public health, with only a few treatment options and a > 50% fatality rate. Although several resistance mechanisms are understood, it is still impossible to predict which mutations are most likely to occur. Here, we demonstrate that independent samples of Ab, exposed to different carbapenems with escalating concentrations, show concentration- and carbapenem-dependent trends in β-lactamase-isoform expression. This result, based on the isoforms identified through label-free-quantification LC-MS/MS measurements of cell-free, gel-separated β-lactamases, suggests that the appearance of antibiotic resistance may be have some predictability based on structural factors. Additionally, the identified minor isoforms also have high sequence similarity to major expressed isoforms, indicating a potential path over which resistance occurred in independent samples. Antibiotic resistance maybe therefore somewhat predictable based on specific structural properties and further investigation may lead to new strategies for mitigating antibiotic resistance.https://doi.org/10.1038/s41598-024-81501-z |
| spellingShingle | Bogdan M. Benin Trae Hillyer Noah Aguirre Yuk Yin Sham Belinda Willard Woo Shik Shin Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii Scientific Reports |
| title | Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii |
| title_full | Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii |
| title_fullStr | Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii |
| title_full_unstemmed | Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii |
| title_short | Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii |
| title_sort | carbapenem induced β lactamase isoform expression trends in acinetobacter baumannii |
| url | https://doi.org/10.1038/s41598-024-81501-z |
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