Application of rps16 Intron and trnL-trnF Intergenic Spacer Sequences to Identify Rengas Clone Riau
<p>Rengas clone Riau has been identified using morphological characters and molecular technique with a <em>psbA-trnH</em> intergenic spacer, however, this method can only determine its taxonomic status at genus level, namely <em>Gluta</em> sp. This study reports applic...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Negeri Semarang
2017-07-01
|
| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/9108 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <p>Rengas clone Riau has been identified using morphological characters and molecular technique with a <em>psbA-trnH</em> intergenic spacer, however, this method can only determine its taxonomic status at genus level, namely <em>Gluta</em> sp. This study reports application two DNA barcodes, i.e. <em>rps16 </em>intron<em> </em>and <em>trnL-trnF</em> intergenic spacer, to identify Rengas clone Riau. The methods included collection of the leaves from Kajuik Lake, total DNA isolation, electrophoresis, PCR (polymerase chain reaction), gel purification and sequencing. The <em>rps16 </em>intron size was 659 bp and the <em>trnL-trnF </em>intergenic spacer was 527 bp. The BLASTn analysis showed that sequences of the <em>rps16 </em>intron and the <em>trnL-trnF </em>intergenic spacer of <em>Gluta</em> sp clone Riau had 100% similarity to those of <em>G. renghas </em>deposited in GenBank. These results were supported by high max score, high total score, query cover = 100%, and E-value = 0. The dendrograms also showed the closest relationship of <em>Gluta</em> sp clone Riau with <em>G. renghas </em>deposited in GenBank compared to other species of <em>Gluta</em>. In conclusion, this study succeeded in identifying Rengas clone Riau as <em>Gluta renghas</em> by using sequences of the <em>rps16 </em>intron and the <em>trnL-trnF</em> intergenic spacer. A combination of DNA barcodes could be applied to identify various plants as long as the database for the DNA barcodes is available in public database such as GenBank. </p> |
|---|---|
| ISSN: | 2085-191X 2338-7610 |