Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of Grapevine
In this study, we carried out large-scale leaf spot symptom observation on vineyards in the Krasnodar Krai of Russia and determined their distribution. The incidence and severity of leaf spot were higher on the Euro-American grapevine hybrids (Bianka, Levokumskij, Avgustin, Moldova, Pervenets Magara...
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2024-11-01
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| author | Evgeniya Yurchenko Daria Karpova Margarita Burovinskaya Svetlana Vinogradova |
| author_facet | Evgeniya Yurchenko Daria Karpova Margarita Burovinskaya Svetlana Vinogradova |
| author_sort | Evgeniya Yurchenko |
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| description | In this study, we carried out large-scale leaf spot symptom observation on vineyards in the Krasnodar Krai of Russia and determined their distribution. The incidence and severity of leaf spot were higher on the Euro-American grapevine hybrids (Bianka, Levokumskij, Avgustin, Moldova, Pervenets Magaracha, Dunavski lazur). A total of 433 isolates that belonged to the genus <i>Alternaria</i> were isolated from samples with leaf spot. Pathogenicity testing confirmed the ability of the representative isolates to cause necrosis on the grapevine. The isolates of <i>Alternaria</i> sp. were typed by the loci of internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (<i>gapdh</i>), Alternaria allergen a1 (<i>Alt a1</i>), β-tubulin (<i>tub</i>), and translation elongation factor (<i>tef1</i>). Isolates from grapevine causing leaf spot were shown to cluster with isolates of <i>Alternaria. alternata</i> (Fr.) Keissl. and <i>Alternaria. arborescens</i> E.G. Simmons species complex. Of the fungicides tested to inhibit <i>Alternaria</i> growth, the most effective were mixtures, such as pyrimethanil and fluopyram, cyprodinil and fludioxonil, and those that included difenoconazole. The results of the study expand our knowledge of the biodiversity of <i>Alternaria</i> sp. fungi and can be used to limit the spread of <i>Alternaria</i> leaf spot of the grapevine. |
| format | Article |
| id | doaj-art-21383a25c864462a969670002ab6e000 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| series | Plants |
| spelling | doaj-art-21383a25c864462a969670002ab6e0002024-12-13T16:30:00ZengMDPI AGPlants2223-77472024-11-011323333510.3390/plants13233335Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of GrapevineEvgeniya Yurchenko0Daria Karpova1Margarita Burovinskaya2Svetlana Vinogradova3North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-Making, 40 Years of Victory Street, Build. 39, 350901 Krasnodar, RussiaNorth Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-Making, 40 Years of Victory Street, Build. 39, 350901 Krasnodar, RussiaNorth Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-Making, 40 Years of Victory Street, Build. 39, 350901 Krasnodar, RussiaSkryabin Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect, 33, Build. 2, 119071 Moscow, RussiaIn this study, we carried out large-scale leaf spot symptom observation on vineyards in the Krasnodar Krai of Russia and determined their distribution. The incidence and severity of leaf spot were higher on the Euro-American grapevine hybrids (Bianka, Levokumskij, Avgustin, Moldova, Pervenets Magaracha, Dunavski lazur). A total of 433 isolates that belonged to the genus <i>Alternaria</i> were isolated from samples with leaf spot. Pathogenicity testing confirmed the ability of the representative isolates to cause necrosis on the grapevine. The isolates of <i>Alternaria</i> sp. were typed by the loci of internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (<i>gapdh</i>), Alternaria allergen a1 (<i>Alt a1</i>), β-tubulin (<i>tub</i>), and translation elongation factor (<i>tef1</i>). Isolates from grapevine causing leaf spot were shown to cluster with isolates of <i>Alternaria. alternata</i> (Fr.) Keissl. and <i>Alternaria. arborescens</i> E.G. Simmons species complex. Of the fungicides tested to inhibit <i>Alternaria</i> growth, the most effective were mixtures, such as pyrimethanil and fluopyram, cyprodinil and fludioxonil, and those that included difenoconazole. The results of the study expand our knowledge of the biodiversity of <i>Alternaria</i> sp. fungi and can be used to limit the spread of <i>Alternaria</i> leaf spot of the grapevine.https://www.mdpi.com/2223-7747/13/23/3335<i>Vitis vinifera</i><i>Alternaria</i> leaf spot of grapevinemultilocus typing<i>Alternaria alternata</i><i>Alternaria arborescens</i>fungicides |
| spellingShingle | Evgeniya Yurchenko Daria Karpova Margarita Burovinskaya Svetlana Vinogradova Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of Grapevine Plants <i>Vitis vinifera</i> <i>Alternaria</i> leaf spot of grapevine multilocus typing <i>Alternaria alternata</i> <i>Alternaria arborescens</i> fungicides |
| title | Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of Grapevine |
| title_full | Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of Grapevine |
| title_fullStr | Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of Grapevine |
| title_full_unstemmed | Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of Grapevine |
| title_short | Leaf Spot Caused by <i>Alternaria</i> spp. Is a New Disease of Grapevine |
| title_sort | leaf spot caused by i alternaria i spp is a new disease of grapevine |
| topic | <i>Vitis vinifera</i> <i>Alternaria</i> leaf spot of grapevine multilocus typing <i>Alternaria alternata</i> <i>Alternaria arborescens</i> fungicides |
| url | https://www.mdpi.com/2223-7747/13/23/3335 |
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