Standardization guidelines and future trends for PET hydrolase research
Abstract Enzymatic depolymerization of polyethylene terephthalate (PET) towards monomer recycling offers a green route to a circular plastic economy, with scale-up currently underway. Yet, inconsistent assessment methods hinder clear comparisons between various PET hydrolases. This Perspective aims...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-05-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60016-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850268669279469568 |
|---|---|
| author | Ren Wei Peter Westh Gert Weber Lars M. Blank Uwe T. Bornscheuer |
| author_facet | Ren Wei Peter Westh Gert Weber Lars M. Blank Uwe T. Bornscheuer |
| author_sort | Ren Wei |
| collection | DOAJ |
| description | Abstract Enzymatic depolymerization of polyethylene terephthalate (PET) towards monomer recycling offers a green route to a circular plastic economy, with scale-up currently underway. Yet, inconsistent assessment methods hinder clear comparisons between various PET hydrolases. This Perspective aims to identify critical gaps in this dynamic research field and outline key principles for selecting and tailoring novel enzymes, such as using uniform PET samples and standardizing reaction settings that mimic industrial conditions. Applying these guidelines will improve enzyme screening efficiency, increase data reproducibility, deepen the understanding of interfacial biocatalysis, and ultimately accelerate the development of more robust and cost-effective bio-based PET recycling methods. |
| format | Article |
| id | doaj-art-10cf7702fc264bb4a99ec7c51279552d |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-10cf7702fc264bb4a99ec7c51279552d2025-08-20T01:53:23ZengNature PortfolioNature Communications2041-17232025-05-0116111310.1038/s41467-025-60016-9Standardization guidelines and future trends for PET hydrolase researchRen Wei0Peter Westh1Gert Weber2Lars M. Blank3Uwe T. Bornscheuer4Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of GreifswaldDepartment of Biotechnology and Biomedicine, Technical University of DenmarkMacromolecular Crystallography, Helmholtz-Zentrum Berlin für Materialien und EnergieiAMB - Institute of Applied Microbiology. ABBt - Aachen Biology and Biotechnology, BioSC, RWTH Aachen UniversityDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of GreifswaldAbstract Enzymatic depolymerization of polyethylene terephthalate (PET) towards monomer recycling offers a green route to a circular plastic economy, with scale-up currently underway. Yet, inconsistent assessment methods hinder clear comparisons between various PET hydrolases. This Perspective aims to identify critical gaps in this dynamic research field and outline key principles for selecting and tailoring novel enzymes, such as using uniform PET samples and standardizing reaction settings that mimic industrial conditions. Applying these guidelines will improve enzyme screening efficiency, increase data reproducibility, deepen the understanding of interfacial biocatalysis, and ultimately accelerate the development of more robust and cost-effective bio-based PET recycling methods.https://doi.org/10.1038/s41467-025-60016-9 |
| spellingShingle | Ren Wei Peter Westh Gert Weber Lars M. Blank Uwe T. Bornscheuer Standardization guidelines and future trends for PET hydrolase research Nature Communications |
| title | Standardization guidelines and future trends for PET hydrolase research |
| title_full | Standardization guidelines and future trends for PET hydrolase research |
| title_fullStr | Standardization guidelines and future trends for PET hydrolase research |
| title_full_unstemmed | Standardization guidelines and future trends for PET hydrolase research |
| title_short | Standardization guidelines and future trends for PET hydrolase research |
| title_sort | standardization guidelines and future trends for pet hydrolase research |
| url | https://doi.org/10.1038/s41467-025-60016-9 |
| work_keys_str_mv | AT renwei standardizationguidelinesandfuturetrendsforpethydrolaseresearch AT peterwesth standardizationguidelinesandfuturetrendsforpethydrolaseresearch AT gertweber standardizationguidelinesandfuturetrendsforpethydrolaseresearch AT larsmblank standardizationguidelinesandfuturetrendsforpethydrolaseresearch AT uwetbornscheuer standardizationguidelinesandfuturetrendsforpethydrolaseresearch |